Journal: European journal of pain (London, England)

Article Title: Hypercapnia Reduces Perceived Heat Pain in Healthy Subjects.

doi: 10.1002/ejp.70001

Figure Lengend Snippet: FIGURE 1 | Schematic of the participant setup. Left side: In-house-built wooden frame (hypercapnibar) with four Douglas bags, two for each mix- ture (normocapnic and hypercapnic) indicated by different colours (dark green and light brown). Bags of the same mixture are connected through three-way directional manual stopcocks, which are themselves connected by tubes to a central stopcock, from which another tube delivers the mix- ture to the oronasal face mask of the participant. Right side: Participant representation with three measuring sites of autonomic recordings. Oronasal face mask with sampling tube to monitor oxygen (O2) and carbondioxide (CO2), three electrodes on the torso (distally below left and below right clavicule and distally above right hip) for electrocardiography (ECG), one pair of electrodes at the left foot (attached to the big and middle toes) for recording galvanic skin response (GSR).

Article Snippet: The four bags were mounted onto a customised, in- house- built wooden frame, the so- called hypercapnibar (height 125 cm, width 152 cm, depth 11.5 cm, plank thickness 3 cm), which had three 3- way directional manual stopcocks with a 2.86 cm inner port diameter (ADInstruments, Dunedin, NZ) screwed onto the top plank, one in the centre and one at each end.

Techniques: Sampling

Journal: bioRxiv

Article Title: Reactive Oxygen Detoxification Contributes to Mycobacterium abscessus Antibiotic Survival

doi: 10.1101/2024.10.13.618103

Figure Lengend Snippet: (A) M. smegmatis (MC 155), (B) M. tuberculosis (Erdman), or (C) M. abscessus (ATCC 19977) were starved in PBS or grown in 7H9 rich media. The indicated antibiotics were added: Isoniazid (INH), rifampin (RIF), ethambutol (EMB) tigecycline (TIG) and linezolid (LZD). Surviving colony forming units (CFUs) are shown. Error bars represent SEM, statistical significance is calculated at each time point using student’s t test. ****: p<0.0001, ***: p<0.001, **: p<0.01, *: p<0.05, ns: p>0.05. Data are combined from 3 independent experiments.

Article Snippet: Antibiotics were used at the following concentrations: tigecycline (Chem-Impex) at 1.25ug/ml (8 fold above MIC, re-administered every 3 days), linezolid (Chem-Impex) at 2.5ug/ml (8 fold above MIC), rifampin (Sigma) at 32ug/ml (8 fold above MIC, re-administered every 6 days), isoniazid (Sigma) at 32ug/ml (8 fold above MIC, re-administered every 6 days), and ethambutol (Thermo) at 4ug/ml (8 fold above MIC, re-administered every 3 days).

Techniques: